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KMID : 0368419980410040318
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Journal of Plant Biology
1998 Volume.41 No. 4 p.318 ~ p.323
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In Vitro Selection of Groundnut Cell Lines from Cercosporidium Personatum Culture Filtrates and Regeneration of Resistant Plants Through Cell Culture
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Venkatachalam, P.
Geetha, N./Jayabalan, N./Saravanababu, S.
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Abstract
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Cell suspensions derived from immature leaves of the groundnut (Arachis hypogaea L.) were cultured in the presence and absence of Cercosporidium personation pathotoxic culture filtrates. Cell viability and reactions of cell lines were determined after exposure to various concentrations (25-100%, v/v) of the filtrates. Cell lines have been selected for resistance to the toxin(s) produced by C. personatum. Selected cell lines were used for plant regeneration on regeneration media containing C. personatum culture filtrates. Plant regeneration frequency was found to be low in long-term cultures, whereas it was high in short-term cultures. The selfed progeny of the plants regenerated from the resistant cell lines showed resistance to the pathogen in the field. Six out of 82 plants exhibited enhanced resistance in the R_2 generation. The culture filtrate stimulated callus proliferation as well as plant regeneration at lower concentrations, a response that could prove to be very useful for obtaining disease resistant plants through in vitro selection.
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